HPLC法同时测定正胃胶囊中没食子酸和橙皮苷的含量

Simultaneous Determination of Gallic Acid and Hesperidin in Zhengwei Capsules by HPLC

目的建立HPLC法同时测定正胃胶囊中没食子酸和橙皮苷含量。方法采用InertsilODS-3 C18(4.6 mm×250 mm,5μm)色谱柱;流动相为甲醇(A)-0.1%磷酸水溶液(B)系统,梯度洗脱;流速为1.0 ml·min-1;检测波长:272 nm(0~8min,没食子酸),283 nm(8~45 min,橙皮苷)。结果没食子酸进样量在0.078~1.409μg范围内线性关系良好(r=0.9999,n=6),平均回收率为99.26%,RSD为1.5%(n=9);橙皮苷进样量在0.051~0.926μg范围内线性关系良好(r=0.9998,n=6),平均回收率为99.32%,RSD为1.6%(n=9)。结论该方法简便、快捷、准确,可用于该制剂的质量控制。

Objective To establish an HPLC method for determination of gallic acid and hesperidin in Zhengwei capsules. Methods Inertsil ODS-3 C18( 4. 6 mm × 250 mm,5 μm) column was used. The mobile phase was methanol-water containing 0. 1% phosphoric acid in the gradient elution mode and at a flow rate of 1. 0ml·min-1. The detection wavelength was 272 nm( 0-8 min) for gallic acid and 283 nm( 8-45 min) for hesperidin. Results Gallic acid had a good linearity within the range of 0. 078-1. 409 μg. The average recovery was99. 26% with an RSD of 1. 5%( n = 9). Hesperidin had a good linearity within the range of 0. 051-0. 926 μg.The average recovery was 99. 32% with an RSD of 1. 6%( n = 9). Conclusion This method is simple,accurate and reproducible. It can be used to determine gallic acid and hesperidin in Zhengwei capsules under the same chromatographic condition.