摘要
通过不同溶剂对羊栖菜有效成分进行提取,并采用DPPH(二苯代苦味酰自由基)体系、羟基自由基体系、烷基自由基引发的亚油酸氧化体系、超氧阴离子自由基体系对各提取物的抗氧化性进行研究,并同VE、TBHQ(特丁基对苯二酚)、茶多酚进行了比较,结果得出羊栖菜粗提物只在DPPH体系和烷基自由基引发的亚油酸氧化体系中具有清除作用,其中以0 5%Na2CO3(W/V)为溶剂的提取物抗氧化能力最好,而以DPPH体系作为检测系统最为稳定。
The effective components were extracted from Sargassum Fusiforme Serchel by various solvents.Using the assay system of DPPH,hydroxyl radical system,oxidation system of linolic acid induced by alkane radical,superoxide radical system,the antioxidation activities of various extracts were studied and compared with tea polyphenol,VE and TBHQ.The results showed that Sargassum Fusiforme Serchel extracts in DPPH system and oxidation system of linolic acid induced by alkane radical have scavenging effect.With 0.5% NaCO3(w/v),the highest exaction ratio and optimal antioxidant activity were reached.The DPPH system is the most stable and effective assay system.
基金
浙江省海洋开发重点攻关项目(01-01)
关键词
羊栖菜提取物
药用
食用
自由基
清除能力
Sargassum fusiforme serchel
antioxidant system
free radical scavenging
