高效液相色谱法测定金茵利胆颗粒绿原酸含量

Study on Quality Control of Jinyin Lidan granule

目的：建立HPLC法测定金茵利胆颗粒绿原酸含量。方法：采用色谱柱：C18（ID 5.0mm ×25cm，4.6μm）；流动相乙腈-0.4%磷酸溶液（10：90）；检测波长为327nm，柱温为30℃，流速为1.0ml/min。结果：以绿原酸进样量C（μg）为横坐标，峰面积A为纵坐标，绘制标准曲线，求得回归方程：A=3.14484×106C＋208.5,（R2=1.000），绿原酸在0.00535～2.140μg范围内线性关系良好(R2=1.000)，平均回收率为99.68%，RSD为0.42%。结论：该方法结果准确，可作为金茵利胆颗粒质量控制的定量方法。

To establish a HPLC method for the determination of chlorogenic acid in Jinyin Lidan granule. Meth-ods:The chlorogenic acid chromatographic experiments were performed on C18 column(5.0mm×250mm, 4.6μm), the mobile phase was acetonitrile-0.4%phosphoric acid (10:90);the detection wavelength was 327nm, column tempera-ture was 30℃, the flow rate was 1.0 ml/min. Results: The linear equations were A=3.14484 × 106C +208.5,(R2=1.000)for hlorogenic acid The linear range of chlorogenic acid was 0.00535~2.140μg(R2=1.000),the average recov-ery was 99.68%with RSD of 0.42%. Conclusions:The method is feasible, which can contro1 the quality of Jinyin Lidan granule.