卵巢癌患者血清miR-210、miR-126与临床病理特征及预后关系探讨

The study on relationship between serum MiR-210 and MiR-126 levels with clinicopathological features or prognosis of patients with ovarian cancer

目的:研究卵巢癌患者血清微小RNA-210(miR-210)和微小RNA-126(miR-126)表达水平及其与患者临床病理特征及预后的关系。方法:选取102例本院确诊的卵巢癌患者作为观察组,另选取同期本院健康体检的女性志愿者102例为对照组。采用qRT-PCR法检测两组血清miR-210、miR-126相对表达量,根据检测中位值将miR-210、miR-126分为高表达组与低表达组,观察其与患者临床病理特征的关系,受试者工作特征曲线(ROC)法分析miR-210、miR-126表达对卵巢癌的预测价值,Kaplan-Meier分析卵巢癌患者生存,logistic多元回归分析影响卵巢癌发生的危险因素。结果:与对照组相比,观察组血清miR-210表达水平升高,miR-126表达水平降低(P<0.05);miR-210、miR-126均与患者淋巴结转移、FIGO分期、分化程度及糖类抗原125水平相关,且miR-210与组织类型相关(P<0.05);miR-210、miR-126的曲线下面积分别为0.789、0.836,敏感度分别为96.6%、67.8%,特异度分别为51.2%、95.4%;miR-210低表达组无生存进展期(PFS)、总体生存时间(OS)均高于高表达组,miR-126高表达组PFS、OS均高于低表达组(P<0.05);miR-210、miR-126均为影响卵巢癌发生的独立危险因素。结论:卵巢癌患者的血清miR-210上调表达, miR-126下调表达,均与卵巢癌发生发展有关,均可能作为临床诊断、监测病情及评估预后的重要指标。

Objective: To study the expression levels of serum micro RNA-210(miR-210) and micro RNA-126(miR-126) of patients with ovarian cancer, and to explore the relationships between the levels of miR-210 and miR-126 and the clinicopathological features and prognosis of the patients. Methods: 102 patients with ovarian cancer were selected in study group, and another 102 healthy female volunteers were selected in control group. The expression levels of serum miR-210 and miR-126 of women in the two groups were detected by qRT-PCR, and women in study group were divided into high expression group and low expression group according to the median value of the levels of serum miR-210 and miR-126. Receiver operating characteristic curve(ROC) was used to analyze the predictive values of miR-210 and miR-126 expressions, Kaplan-Meier was used to analyze the survival of patients with ovarian cancer, and Logistic multiple regression analysis was used to analyze the risk factors of ovarian cancer. Results: Compared with the control group, the expression level of serum miR-210 of patients in study group increased(P<0.05), but the expression level of miR-126 was decreased significantly(P<0.05). MiR-210 and miR-126 levels were significantly correlated with lymph node metastasis, FIGO stage, degree of differentiation, and the serum carbohydrate antigen 125(CA125) level of patients(P<0.05), and miR-210 level was significantly correlated with histological type(P<0.05). ROC analysis showed that the area under curve(AUC) of miR-210 level and miR-126 level were 0.789 and 0.836, respectively, their sensitivity were 96.61% and 67.80%, respectively, and their specificity were 51.16% and 95.35%, respectively.