摘要
利用通用载体质粒融合系统UPS (univectorplasmid -fusionsystem)构建出了以GFP (绿色荧光蛋白 )为报告基因的酵母表达载体 ,经PCR、电泳、测序等手段证明了构建的准确性 。
S. cerecisiae expression vector was constructed by UPLS(univector plasmid-fusion system) with GFP as report gene. By the way of PCR, electrophoresis and sequence measurement and so on, the result showed accuracy of construction, and simultaneously, high efficiency and convenience of UPS were also testified.
出处
《昆明医学院学报》
CAS
2003年第1期13-16,共4页
Journal of Kunming Medical College
基金
国家自然科学基金资助 (39880 0 4 4)
广州市重大科技项目资助 (199- 2 0 0 5 - 0 0 1)