白色念珠菌天冬氨酸蛋白酶与菌株毒力的研究

The Role of Candida Albicans Secreted Aspartic Proteinase in Virulence

目的 :观察分泌型天冬氨酸蛋白酶 (secretedasparticproteinase ,SAP)与念珠菌致病性的关系 .方法 :从住院病人中分离培养出 5 0株念珠菌 ,鉴定菌种后采用限制氮盐的培养基半定量测定天冬氨酸蛋白酶 ,并筛选蛋白酶阳性或阴性菌株 .经证实为系统感染的 10株菌株腹腔感染健康小鼠 .结果 :白色念珠菌天冬氨酸蛋白酶阳性率高达 90 % ,类星型和伪热带次之 .蛋白酶阳性或阴性的念珠菌对健康小鼠均无致病性 .结论

To investigate the correlation between candida albicans secreted aspartic proteinase and stain virulence. Methods: 50 strains of candida from patients in our hospital. The strains were identified by assimilation reaction. The enzyme was determined by semi-quantitative, which was on albumin agar plate Nitrogen-restricted media. The strains that can lead to systemic inflammation were applied to infect healthy kunming mice. Result: 90% candida albicans can produce this type of enyme. Enzyme-produced and non-enzyme-produced candida albicans were both not pathogenetic to this mouse. Conclusion: In general condition, aspartic proteinase can not increase the pathogenesis of candida albicans.