摘要
目的建立改良大鼠舌下静脉采血法,以更好更快地实现实验大鼠多次、安全、高质量的采血。方法以10%水合氯醛溶液腹腔内注射麻醉大鼠后,充分暴露舌下静脉,采用一次性负压管进行舌下静脉取血。结果经过改良后的舌下静脉采血方法,即使是初学者也可轻松收集到2~3 m L的血液样本,并且采血间隔时间短,对大鼠日常活动无显著影响。结论改良后的舌下静脉采血符合动物福利原则,对于初学者来说是大鼠活体采血的首选方式,操作便捷样本质量高且对大鼠损伤相对较小。
Objective To achieve a method of multiple,safe and high quality blood collection in rats with the improved sublingual vein blood sampling. Methods After anesthetized the rats with intraperitoneal injection of 10% chloral hydrate solution,the hypoglossal vein was fully exposed and the blood was collected by disposable negative pressure tubes. Results The improved method of sublingual venous blood collection can easily collect blood samples of 2 to 3 m L,and the interval of blood collection was short with no significant effect on daily activities of rats. Conclusion The improved hypoglossal vein blood collection is the first choice for the beginners,and it is most in line with the principle of animal welfare. It is easy to gain high quality samples with relatively less damage to rats.
作者
安琪
傅龙龙
张长勇
谷翊群
葛争艳
AN Qi;FU Long-long;ZHANG Chang-yong;GU Yi-qun;GE Zheng-yan(Graduate School of Peking Union Medical College,Beijing 100730,China;Department of Male Clinical Research National Research Institute for Family Planning/WHO Collaborating Center for Research in Human,Beijing 100081,China;Animal Experiment Center of Family Planning/WHO Collaborating Center for Research in Human,Beijing 100081,China;Institute of Basic Medicine,Xiyuan Hospital of Chinese Academy of Chinese Medical Sciences,Beijing 100091,China)
出处
《局解手术学杂志》
2019年第3期250-252,共3页
Journal of Regional Anatomy and Operative Surgery
基金
中央高校基本科研业务费专项资金(3332018187)
中央级公益性科研院所基本科研业务费专项(2015GJZ08)
关键词
大鼠
舌下静脉
采血
动物福利
experiment rats
sublingual vein
blood collection
animal welfare