用不同类型的水稻鉴别品种鉴定江苏稻瘟病菌的致病性

Study on the Pathogenicity of Magnaporthe grisea Collected from Jiangsu with Four Sets of Differentials

根据 DNA指纹图谱和系谱 ,从 1999年采集的 96个江苏稻瘟菌株中选择 44个菌株进行致病性测定。它们被鉴定为 7个生理小种。用日本清泽系列单基因鉴别品种和 L TH近等基因系 (NIL s)鉴别品种分析 ,分别产生 2 7和 10个致病类型 ,CO39NIL s对江苏菌株几乎没有鉴别作用。分析结果可见供试菌株之间致病性差异极大。研究表明 ,发展包含较多抗病基因的近等基因系鉴别品种能准确地反映病原菌致病性以及合理评价和利用相关抗性基因。

representative isolates of M.grisea were chosen from 96 isolates collected from rice in Jiangsu in 1999 according to rep-PCR fingerprints, host varieties and collection areas. The pathogenicity was studied with 4 sets of differentials. The results indicated that the Chinese race differentials, the Tsunematru mono-resistance-gene lines from Japan and the LTH-NILs could differentiate the 44 isolates into 7, 27 and 10 pathogenic types respectively, while CO39 NILs showed poor differentiating ability. The results also revealed that there existed many differences in the scope of the pathogenicity of different isolates. It is suggested that the inclusion of improvement of more resistance genes into the NIL differentials might lead to more accurate appraising of the pathogenicity of the pathogen and resistance genes of the rice varieties.