大麦BYDV抗性的印迹杂交分析

Dot-blot Hybridization Analysis of Resistance to BYDV in Barley

大麦抗黄矮病毒 (BYDV)育种面临的主要困难之一 ,就是常规生物学抗性检测技术不能准确地进行抗性检测和抗性基因筛选。本文在前人的基础上 ,以大麦黄矮病毒 PAV株系和 RPV株系基因组中的特异核酸序列为探针 ,应用斑点印迹杂交分析技术 ,建立了一套简单、快速、有效的 BYDV抗性检测方法和抗性基因 (Yd2 )的筛选方法 ;同时也建立了一套适用于大麦 BYDV抗性检测的较为有效的带毒蚜虫饲养和管理方法。选用了 12个已知抗性和基因型的大麦品种 (系 )进行抗性检测 ,结果证实了这一抗性检测技术体系的可靠性与有效性。

One of the major difficulties in barley breeding resistant to BYDV is that traditional techniques of biological resistance assays can not perform resistance assays and resistant genes screening accurately. Using specific nucleic acids sequence derived from the genomes of BYDV-PAV and BYDV-RPV strains as the probes and the method of Dot-blot hybridization analysis, the authors developed a set of simple, quick and efficient methods on BYDV resistant assay and screening of resistant gene(Yd 2) on the basis of precursors. Meanwhile, a system of viruliferous aphids raising and management was also established. Finally, the authors confirmed the reliability and efficiency of the resistance assay system using 12 barley cultivars with known resistance and genotypes.