血管紧张素Ⅱ1型受体基因多态性与高血压左室重构的相关性研究

Relationship between angiotensin II type 1 receptor gene polymorphism with left ventricular hypertrophy in patients with hypertension

目的 :探讨血管紧张素Ⅱ 1型受体 (AT1R)基因A116 6C多态性与原发性高血压 (EH)及其左室重构的关系。方法 :测定 10 4例EH患者和 15 4例健康对照者的血压 ,身高 ,体重 ,空腹血糖、总胆固醇、甘油三酯浓度 ;测定EH患者的左室重量指数 (LVMI)并记录病程 ;多聚酶链式反应 限制性酶切法 (PCR RFLP)鉴定AT1R基因 116 6位点基因型。结果 :EH组AT1R基因AC/CC基因型频率高于对照组 (0 .2 0 2∶0 .0 97,P <0 .0 5 ) ,116 6C等位基因频率高于对照组 (0 .115∶0 .0 5 2 ,P <0 .0 1) ;用协方差分析排除病程、收缩压、总胆固醇等混杂因素的作用后发现 ,EH组中AC/CC基因型患者LVMI高于AA基因型者 (12 7.37∶115 .98,P <0 .0 5 )。结论 :AT1R基因 116 6位点AC/CC基因型及C等位基因与EH及其左室重构有关。

Objective:To investigate the relationships between hypertension, hypertensive left ventricular remodeling with polymorphism of angiotensin II type 1 receptor (AT 1R) gene.Methods:Blood pressure, body mass index, fasting serum glucose, serum total cholesterol and triglyceride were measured in all subjects, including 104 hypertensives patients and 154 normotensives health persons. Left ventricular mass index were measuned by echocardiogram. Polymerase chain reaction combined with restriction enzyme digestion was used to determine the polymorphism of the 1166 site of AT 1R gene. Analyze the frequencies of AC/CC genotypes and 1166C allele of AT 1R gene between hypertensives and 154 normotensives by Chi Square test. Multiple linear regression was used to analyze the linear relation between the clinical data and LVMI. Results:The frequencies of AC/CC genotypes and 1166C allele of AT 1R gene in hypertensives patients were higher than that in control group (AC/CC genotypes 0.202 ∶ 0.097 ,P< 0.05 ; C allele 0.115 ∶ 0.052 ,P< 0.01 ). When covariant factors were rejected ( 127.37 ∶ 115.98 , P< 0.05 ), LVMI was higher in patients with AC/CC genotypes than that in patients with AA genotypes. Conclusion:1166 AC/CC genotypes and 1166C allele were associated with hypertension and hypertensive left ventricular remodeling.