一步法检测AFB和斑点法检测LAM-IgG诊断结核性脑膜炎

COMBINED DETECTION OF AFB AND LAM-IgG BY"SINGLE STEP AFB DETECTION" AND"DOT IGFA"IN CSF FOR DIAGNOSIS OF TUBERCULOUS MENINGITIS

目的 :评估联合应用一步法检测抗酸杆菌 (AFB)和斑点法检测抗阿拉伯糖甘露糖脂 Ig G抗体(L AM- Ig G)方法诊断结核性脑膜炎 (结脑 )的价值。方法 :结脑患者为实验组 ,采集脑脊液本本 (CSF)为标本 ,应用一步法检测 AFB、斑点法检测 LAB- Ig G。结果 :实验组 ,一步法检测 AFB阳性率为 6 7.4 %(6 2 /92 )、斑点法检测 LAM- Ig G阳性率为 5 7.6 % (5 3/92 )、两者联检的阳性率为 71.7% (6 6 /92 ) ,较常规方法的阳性率有显著性提高 (ρ<0 .0 0 1)。结论 :对 CSF标本联合应用一步法检测 AFB和斑点法检测LAM- Ig G。

Objctive:To appraise the value on combined detection of acid fast bacilli(AFB)and Lipoarabionomannan G antibody(LAM IgG) in cerebrospinal fluid(CSF)for diagnosis of tuberculous meningitis.Methods:The test group was the patients with tuberculous meningitis.AFB was detected in CSF with'single step AFB detection'and LAM IgG was detected in CSF with'dot IGFA'.Results:The positive rate of AFB was 67.4%(62/92)and LAM IgG was 57.6%(53/92) and AFB+LAM IgG was 71.7%(66/92) in tuberculous meningitis group.Conclusion:AFB and LAM IgG are useful index to diagnose tuberculous meningitis.Combined using of them can improve the sensitivity.