高表达FasL的Sertoli细胞在睾丸局部感染时的免疫调节作用

Immune regulation of Sertoli cells overexpressing FasL in the local infection of testis

目的 研究高表达FasL的转基因小鼠Sertoli细胞在睾丸局部感染时的免疫调节作用。方法 以溶脲脲原体(Ureaplasmaurealyticum ,UU)直接注入FasL转基因的小鼠膀胱模拟上行性感染的途径 ,分别在 1、2和 3周处死小鼠 ,分离取得睾丸组织 ,观察组织的病理变化 ,并从小鼠睾丸组织分离获得高纯度的Sertoli细胞 ,然后抽提总RNA ,用RT PCR方法比较正常组与UU感染组之间FasL、IL 1α、TGF βmRNA的表达差异。 结果 与正常组相比 ,UU感染的小鼠 ,睾丸组织发生明显的病理改变 ,Sertoli细胞表达的调节因子在mRNA水平也发生了明显的变化 :其FasLmRNA的表达在 1周 ,3周时与正常无明显区别 ,2周时明显升高 ;IL 1α、TGF βmRNA的表达在 1～ 3周均为升高。

Objective To investigate the immune regulation of Sertoli cells overexpressing FasL in the local infection of FasL trans genic mouse' s testis. Methods Ureaplasma urealyticum(UU) were directly inj ecte d into bladder, which mimicked an ascending infectious way, and at the same tim e the culture medium of UU was injected into another mouse's bladder in control group. Then the mice were put to death at week 1,2 and 3 after injection respect ively. Then pathological alterations in testis were analyzed by histological exa mination. Sertoli cells were also separated from these mice's testis, the compar ison of levels of FasL,IL 1α and TGF β mRNA expression among control and UU infected groups was made by RT PCR.Results As compared with control group, the testis from UU infected mice exhibited lots of morphological abnormalities. In addition, the levels of IL 1α,TGF β mRNA expression of UU infected groups increased significantly after infection from one to three week s, especially at week 2.But level of FasLmRNA expression in UU infected groups only showed a great rise at week 2.Howe ver , at week 1 and week 3,there wer e no significantly difference between control and infected groups.Conclusions During anti infectious immunity transgenic mouse's Sertoli cells whic h express FasL highly may regulate immune function of the testis by the aid of c hanges the secretion of relevant cytokines. [