摘要
目的 :探讨原发性肝癌 (HCC)中p5 3基因的缺失频率、方式、特点及其临床意义。方法 :以p5 3基因及 17号染色体着丝粒DNA为探针 ,应用间期双色荧光原位杂交 (interphasedual,FISH)技术。结果 :肝癌中p5 3基因的缺失频率为 68 0 % (68/ 10 0 ) ,而配对正常肝组织中则未见有p5 3缺失 ;p5 3缺失方式多样且往往伴随 17号染色体多倍体 (r=0 5 94,P <0 0 1) ;p5 3缺失与肝癌病人的性别、年龄、HBV感染及临床分期无显著相关 (P >0 0 5 ) ,但与血清甲胎蛋白 (AFP)水平、肿瘤大小则显著相关 (P <0 0 5 ) ;有无p5 3缺失病人之间 2年存活率差异非常显著 (χ2 =11 463 ,P <0 0 1)。结论 :p5 3基因在原发性肝癌中存在高频缺失并往往伴随 17号染色体的多倍体 ;双色FISH技术为评估基因的缺失提供了特异、敏感、直观的分子细胞遗传学证据。
AIM: To investigate the frequency and pattern of deletion of p53 gene in primary hepatocellular carcinoma (HCC) and its clinical significance. METHODS: The interphase dual fluorescence in situ hybridization(FISH) technique was applied to detect loss of p53 gene in HCCs. RESULTS: The deletion of p53 gene was found in 68.0% of HCCs whereas no loss of p53 gene was detected in 40 mated normal liver specimens. Loss of p53 gene was closely related to tumor size and serum α-fetoprotein(AFP) level in HCC patients ( P< 0.05) but not to HCC patients' sex, age, HBV infection and clinical staging ( P> 0.05). The 2-year survival rate of postoperative HCC patients was significantly lower in the HCC cases with p53 gene deletion (25.6%) than those without p53 gene loss (69.6%) ( χ 2=11.463, P< 0.01). CONCLUSIONS: There is a high frequency of the deletion of p53 gene in primary HCCs. Dual FISH technique provides specific, sensitive and direct evidence for molecular cytogenetic evaluation of gene deletion in tumors.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2003年第3期306-309,T003,共5页
Chinese Journal of Pathophysiology
基金
国家杰出青年B类基金资助项目 (3 982 5 5 11)
广东省自然科学基金资助项目 (980 10 7)
关键词
原发性肝癌
P53基因
高频缺失
原位杂交
Liver neoplasms
Genes, p53
In situ hybridization
Chromosomes, human, pair 17