迟发型超敏反应中小鼠脾淋巴细胞与细胞外基质粘附能力的变化

Adhesion of mice lymphocytes to extracellular matrix in delayed-type hypersensitivity

目的 :探讨迟发型超敏反应中淋巴细胞与细胞外基质粘附能力的变化及细胞因子的调节作用。方法 :以 2 ,4,6-三硝基氯苯 (picrylchloride ,PCl)两次致敏小鼠后 ,在耳部攻击造成超敏反应。取攻击后不同时间点的脾淋巴细胞 ,以Mn2 + 为诱发剂 ,检测细胞与细胞外基质蛋白的粘附活性变化 ;或取攻击后 18h的脾淋巴细胞 ,分别在体外与IL - 2 ,IFN -γ ,TNF -α单独或联合培养 4h后 ,检测细胞与细胞外基质的粘附作用 ;将脾淋巴细胞纯化为T细胞后 ,检测TNF -α对其粘附能力的影响。结果 :脾淋巴细胞与细胞外基质的粘附能力在攻击后 6h时开始上升 ,18h达到高峰 ,之后下降 ,在 3 6h基本恢复正常水平。IL - 2在 10× 10 4 U/L显著促进脾淋巴细胞的粘附 ,TNF -α剂量依赖性地促进脾淋巴细胞的粘附 ,IFN -γ对TNF -α的作用具有明显的协同效应。TNF -α对脾T细胞粘附能力的促进作用强于对总脾淋巴细胞的作用。结论 :迟发型超敏反应中 ,T淋巴细胞与细胞外基质的粘附随炎症的进展发生相应的变化 。

AIM: To investigate the adhesion between lymphocytes and extracellular matrix (ECM) in delayed-type hypersensitivity (DTH) in mice as well as the regulatory effect of several cytokines on it. METHODS: DTH was induced by the application of picryl chloride (PCl) to mice that had been sensitized twice by PCl, followed by isolating the spleen lymphocytes at different times. The adhesion of spleen lymphocytes to fibronectin or typeⅠcollagen were examined. In some cases, spleen lymphocytes obtained at 18 h were incubated with various concentrations of IL-2, IFN-γ, and TNF-α for 4 h at 37℃ before the adhesion assay. T cells purified from the spleen lymphocytes were also used for the assay in the presence of TNF-α. RESULTS: The adhesion of spleen lymphocytes to ECM began to increase at 6 h after PCl challenge, reached a peak at 18 h, then decreased and returned to normal level at 36 h. IL-2 significantly increased the adhesion of lymphocytes to ECM at a dose of 1×10 5 U/L and TNF-α enhanced the adhesion in a concentration-dependent manner. The combined use of TNF-α and IFN-γ showed a significantly enhanced adhesion. TNF-α was more effective to accelerate the adhesion of spleen T Cells than that of total spleen lymphocytes. CONCLUSION: In the process of DTH, the adhesion of lymphocytes to ECM was regulated by several cytokines and associated with the development of inflammation.