四逆汤对家兔动脉粥样硬化的形成及血管壁神经酰胺含量的影响

Effects of Sini decoction on atherosclerosis and ceramide content of aorta in rabbits

目的 :观察四逆汤预防性用药 ,对家兔实验性动脉粥样硬化的形成和血管壁神经酰胺量的影响 ,探讨四逆汤抗AS的作用及可能机制。方法 :将 2 8只新西兰家兔随机分为 3组 :正常对照组、高胆固醇组、四逆汤 +高胆固醇组。分别给予普通饲料 (正常对照组 )和高胆固醇饮食 (其它 2组 )喂养 ;四逆汤 +高胆固醇组另预防性给予四逆汤方药 ,实验结束后 ,运用图象分析法测定主动脉内膜脂质斑块面积百分比 ;用高效薄层层析扫描分析血管组织神经酰胺的变化 ;用TUNEL法检测血管组织细胞的凋亡指数。结果 :四逆汤可明显缩小主动脉内膜脂质斑块面积 ,降低动脉组织神经酰胺浓度 ,减少血管壁细胞凋亡的数量。结论 :四逆汤具有较好的抗AS作用 。

AIM: To observe the effects of Sini decoction on atherosclerosis(AS) and ceramide content of aorta in rabbits. METHODS: 28 rabbits were randomly divided into three groups. Control group was fed with a normal diet; High cholesterol group was fed 1% cholesterol and 5% fat diet; Sini decotion+ high cholesterol group was fed 1% cholesterol and 5% fat diet plus Sini decotion (4.2 g·kg -1 ·d -1 ). At the end of study, the plaque area were measured, the atorta ceramide and cell apoptosis were also detected. RESULTS: Sini decotion diminished lipid plaque area on the aortic endothelium, reduced the levels of aorta ceramide and the apoptosis index. CONCLUSION: Sini decoction has an inhibitory effect on AS, the mechanism may be that Sini decotion reduces concentration of ceramide in aorta.