病毒性心肌炎脾脏淋巴细胞的活化与凋亡

Activation and apoptosis of spleen lymphocytes in patients with viral myocarditis

目的 :探索病毒性心肌炎 (VMC)发病的脾脏机制。方法 :运用DNA缺口末端标记法、免疫组化技术检测实验组 (VMC猝死者 ,8例 )及对照组 (非心性死亡者 ,4例 )脾组织中细胞凋亡及主要组织相容性复合物 (MHC)Ⅱ类抗原的表达情况。结果 :实验组脾组织内可见大量的凋亡细胞 ,脾组织MHCⅡ类抗原高表达 ,动脉周围淋巴鞘MHCⅡ类抗原阳性细胞显著多于对照组 ;对照组脾组织内罕见凋亡细胞 ,MHCⅡ类抗原阳性细胞较少 ,主要位于动脉周围淋巴鞘外围。结论 :VMC患者脾脏免疫细胞的活化与凋亡相伴存在 。

AIM: To explore the role of activation and apoptosis of splenic lymphocytes in the development of viral myocarditis (VMC). METHODS: Apotosis and MHC II antigen of splenic lymphocytes were detected in the VMC group (VMC, death, 8 cases) and control group (non-cardiac death, 4 cases) with TUNEL and immunohistochemistry methods.RESULTS: Compared with the control group, the increased expression of MHC II antigen and apoptosis were found in the splenic lymphocytes in the VMC group. CONCLUSION: These results suggest that the abnormality of the apoptosis/activation of splenic lymphocytes may be involved in the pathogenesis of VMC.