1,6-二磷酸果糖抑制阿霉素致大鼠心肌细胞凋亡的实验研究

Inhibitory effect of fructose-1, 6-diphosphate on adriamycin-induced cardiomyocyte apoptosis in rats

目的 :探讨 1,6-二磷酸果糖 (FDP)对阿霉素 (ADM)导致大鼠心肌细胞凋亡的影响。方法 :2 4只Wis tar大鼠随机分为 3组 :对照组、ADM组、FDP干预组。用比色法测定心肌组织的丙二醛 (MDA)含量、NO-2 /NO-3含量、谷胱甘肽过氧化物酶 (GPx)活性、超氧化物歧化酶 (SOD)活性 ;用TUNEL法检测心肌细胞凋亡 ;用原位杂交法检测心肌细胞的诱导型一氧化氮合酶 (iNOS)mRNA、Bcl- 2mRNA、BaxmRNA表达。结果 :FDP干预组心肌组织的NO-2 /NO-3含量、MDA含量及心肌细胞的iNOSmRNA表达水平、BaxmRNA表达水平、凋亡数量均明显低于ADM组 (P <0 .0 1) ,而其心肌组织的SOD活性、GPx活性及心肌细胞的Bcl- 2mRNA表达水平均明显高于ADM组 (P <0 .0 1)。结论 :FDP拮抗ADM所致心肌细胞的Bcl- 2mRNA表达降低、BaxmRNA表达增加而抑制ADM导致心肌细胞凋亡。

AIM: To study the effect of fructose-1, 6-diphosphate (FDP) on adriamycin(ADM)-induced cardiomyocyte apoptosis in rats. METHODS: Twenty-four Wistar rats were randomly divided into three groups: control group, ADM treated group and FDP intervention group. The contents of malondialdehyde (MDA) and NO - 2/NO - 3, the activities of glutathione peroxidase (GPx) and superoxide dismutase (SOD) were determined by colorimetric method in myocardial tissue, and the cardiomyocyte apoptosis was detected by TUNEL method in myocardial tissue, and the expression of inducible nitric oxide synthase (iNOS) mRNA, Bcl-2 mRNA and Bax mRNA in myocardial tissue were detected by in situ hybridization. RESULTS: The contents of NO - 2/NO - 3 and MDA in myocardial tissue, the expressive levels of iNOS mRNA and Bax mRNA in cardiomyocyes and its apoptotic amounts in FDP intervention group were significantly lower than those in ADM treated group ( P< 0.01). However, the activities of SOD and GPx in myocardial tissue, the expressive level of Bcl-2 mRNA of cardiomyocytes in FDP intervention group were significantly higher than those in ADM treated group ( P< 0.01). CONCLUSION: FDP antagonized the reduced expression of Bcl-2 mRNA and increased expression of Bax mRNA in myocardial tissue induced by ADM, and in turn inhibited ADM-induced cardiomyocyte apoptosis.