不同共作模式的稻田土壤细菌群落结构分析

Analysis of Soil Bacterial Community Structure in Different Paddy Field Co-Cropping Models

为分析不同共作模式的稻田土壤细菌群落结构,利用高通量测序技术,在水稻结穗期对不同共作模式的稻田土壤细菌进行测序,研究稻田单作(SDKBZ)、河蟹共作稻田(SDHXZ)和龙虾共作稻田(SDLXZ)表层土壤细菌群落多样性、丰富度和群落结构的变化。各样本共检测到细菌类群46门、112纲、210目、370科、664属、1 227种。主要的优势菌门是变形菌门(Proteobacteria)、拟杆菌门(Bacteroidetes)、绿弯菌门(Chloroflexi)、放线菌门(Actinobacteria)、酸杆菌门(Acidobacteria)、蓝藻菌门(Cyanobacteria)、Sacharibactaria、疣微菌门(Verrucomicrobia)和厚壁菌门(Firmicutes)。河蟹稻田和龙虾稻田的Chao 1指数均高于单作稻田。PCoA分析结果显示,河蟹稻田和龙虾稻田两种稻田共作模式与稻田单作模式的土壤细菌群落构成差异大。研究初步揭示了河蟹和龙虾与水稻共作复合生态系统具有较丰富的微生物生态组成。

In order to analysis the soil bacterial structure community in different paddy co-cropping models,high throughput sequencing technique was used to study the diversity of bacterial community,richness and changes of community structure in the surface soil of rice field at panicle setting stage for rice field monoculture,river crab paddy field co-cropping and lobster rice field co-cropping.A total of 46 phyla,112 classes,210 orders,370 families,664 genera and 1 227 species of bacteria were detected.The dominant phylum is Proteobacteria,Bacteroidetes,Chloroflexi,Actinobacteria and Acidobacteria.The chao index of crab paddy field and lobster paddy field is higher than that of monoculture rice field.The results of PCoA analysis showed that the composition of soil bacterial community for crab rice field and lobster paddy field was quite different from that of rice field monoculture.The study showed that the ecosystem of rice co-cropping with crab and lobster had richer microbial ecological components.