摘要
目的:建立测定苦参片中氧化苦参碱含量的新方法。方法:用高效液相色谱法,采用 in-ertsil—NH_2柱为分析柱,乙腈—磷酸水溶液(PH=2)—无水乙醇(80:10:12)为流动相,检测波长为215nm,流速为1ml/min。结果:氧化苦参碱在0.02~0.12mg/ml 与峰面积呈良好的线性关系(r=0.9994,n=6),平均回收率为97.98%。结论:该方法简便,快速,重现性好,适用于苦参片中氧化苦参碱的含量测定。
Objective:To develop a new method for the determination of Oxymatrine in Kushen tablets.Method:A HPLC method was set up,using inertsil—NH_2 column.Acetonitrile— water(used phosphoric acid making PH=0.2)—ethyl alcohol(80:10:12)as mobile phase with a flow rate of 1.0ml/min,and detected by UV spectrometry at 215nm.Result:The linear is good in the range of 0.02~0.12mg/ml.The average recovery was 97.98%,while RSD was 1.68%. Conclusion:The method is appropriate for the determination of oxymatrine.
出处
《黑龙江医药》
CAS
2003年第2期87-89,共3页
Heilongjiang Medicine journal