肝硬变时血管活性肠肽肝内清除率的实验研究

The study of hepatic clearance of vasoactive intestinal peptide in cirrhotic rats

目的对肝硬变时血管活性肠肽(vasoactive intestinal peptide,VIP)的肝清除能力进行定量观察。方法采用改良 Miller’s法建立硬变肝脏隔离灌流模型(isolated perfused cirrhotic model,IPCM)行单程灌流(Single-pass perfusion),测定 VIP 的首过肝摄取率及清除率。结果肝硬变时 VIP 肝摄取率(33%±27%)和清除率(8±6ml/min)与正常灌流的肝脏(isolated perfused rat liver,IPRL)无明显差别(P>0.05);但肝脏摄取分数及单位肝重清除率 IPCM(0.06±0.05;0.09±0.25ml·min^(-1)·g^(-1))明显低于 IPRL(0.20±0.13;0.88±0.3ml·min^(-1)·g^(-1))(P<0.01)。结论肝硬变时 VIP 的肝内清除力低于正常肝脏,但肝硬变后肝重和向肝血流量的增加作为一种代偿可部分弥补 VIP 肝清除能力的不足。

Objective Qualitative analysis of hepatic extracting and clearing vasoactive intestinal peptide (VIP) in cirrhotic rats.Methods Isolated perfused rat liver (IPRL) and isolated perfused cirrhotic model (IPCM) were set up and singlepass perfusion mode was used.The hepatic extractions and clearances of VIP were as- sessed.Results In cirrhotics,the extraction (0.06%±0.1%) and clearance (0.39±0.2ml·min^(-1)·g^(-1)) of livers under per gram liver weight and per unit perfusional rate showed a marked decrease as compared with that in the noncirrhotic livers (0.20%±0.1% and 0.88±0.3ml·min^(-1)·g^(-1))(p<0.01).However,there were no significant differences at the total extraction and clearance of livers between IPRLs and IPCMs (P> 0.05).Conclusions A reduced hepatic extractions and clearances of VIP were proved in cirrhosis,but a func- tional compensation is likely due to improving of liver weight and hepatic flow in cirrhosis.