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iQ^(TM)5多重实时荧光定量PCR仪应用及使用方法 被引量:1

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摘要 实时定量PCR(real-time quantitative PCR)通过连续监测PCR扩增指数期间荧光信号强弱变化反应特异性的表达量,并据此计算出目的基因的表达量。此方法不需要分离PCR产物并作为极有效的实验方法,目前已被广泛地应用于分子生物学研究的各个领域。本文主要介绍iQ^(TM)5型荧光定量PCR仪的原理及组成并以植物材料为例,分析介绍仪器的性能、使用方法及操作过程中的注意事项。
出处 《经贸实践》 2016年第2X期310-311,共2页 Economic Practice
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  • 1郭兆奎,魏继承,于艳华,万秀清.烟草转基因检测标准及PCR反应体系的研究[J].中国烟草学报,2000,6(3):18-22. 被引量:10
  • 2Gadani F,Ward M, Guo Z K, et al. Report of the CORESTA task force genetically modified tobacco: detection methods. CORESTA Bulletin, 1999,4:77~148.
  • 3Desjardin L E, Chen Y, Perkihns M D, et al. Comparison of the ABI 7700 system (TaqMan) and competitive PCR for quantitation of Is6110 DNA in Sputum duing treamlent of tuberculosis. J Clin Microbiol, 1998,36:1964~1968.
  • 4De Vries J,Wackemagel W. Detection of nptll gene in genome of transgenic plants by rnarkerrescue transformation. Mol Gen Genet,1998,257:106-113.
  • 5Bustin S A. Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. Journal of Molecular Endocr Endocrinology,2000,25,169-193.
  • 6Yang L, Pan A, Jia J, et al. Validation of a tomato specific gene, LAT52, used as an endogenous reference gene in qualitative and real-time quantitative PCR detection of transgenic tomatoes[J]. J Agric Food Chem(In press).
  • 7Arumuganathan K, Earle E D. Nuclear DNA content of some important plant species[J]. Plant Mol Biol, 1991, 9: 208-218.
  • 8European Commission Regulation (EC) No. 1829/2003 and 1830/2003[J]. Off J Eur Commun L ,2003, 18(268): 1-28.
  • 9Notification No. 2000-31 Ministry of Agriculture and Forestry of Korea, Seoul, Korea . 2000 , 22[Z].
  • 10Notification No. 1775 Food and Marketing Bureau, Ministry of Agriculture, Forestry and Fisheries of Japan, Tokyo, Japan ,2000[Z].

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