摘要
目的 建立通塞脉片中黄芪甲苷含量的测定方法。方法 采用HPLC法 ,Lichrospher5 -C18( 2 5 0mm× 4.6mm)色谱柱 ,流动相为甲醇 :水 ( 76∶2 4) ,流速为 0 .8mL/min ,柱温为 30℃ ,ELSD检测器检测 ,ELSD温度 90℃ ,气体流量 1.5 0SLPM。结果 平均加样回收率为 96 .87%,RSD为 1.71%。结论 方法准确、可靠 ,可用于通塞脉片的质量控制。
OBJECTIVE To establish the method of determination of the content of astragalus glycoside in Tongsaimai Tablet. METHOD HPLC method, Lichrospher5 - C18 (250 mm × 4.6 mm) as the chromatogram column, methanol : water (76:24) as the mobile phase, 0.8 mL/min flow rate, 30℃ column temperature, ELSD for determination, 90℃ ELSD temperature, 1.50 SLPM air flow volume. RESULY The average sample recovery rate was 96.87%, RSD was 1.71%. CONCLUSION This method is accurate and reliable and therefore can be used in the quality control of Tongsaimai Tablet.
出处
《南京中医药大学学报》
CAS
CSCD
2003年第2期94-95,共2页
Journal of Nanjing University of Traditional Chinese Medicine
基金
江苏省科技厅科技攻关项目 (BK2 0 0 12 0 9)
关键词
通塞脉片
高效液相色谱
黄芪甲苷
Tongsaimai Tablet
high-performance liquid chromatography
astragalus glycoside
