去骨瓣减压术对大鼠局灶性脑缺血细胞凋亡的影响

Effect of Decompressive Craniectomy on Changes of Apoptosis after Focal Cerebral Ischemia in Rats

目的:探讨去骨瓣减压术对局灶性脑缺血细胞凋亡的影响。方法:改良Koizumi法制作脑缺血动物模型,6 h后在缺血侧行去骨瓣减压术,采用 TUNEL法测定减压术后4、8、16、24 h皮质和纹状体区细胞凋亡变化,并采用流式细胞仪测定术后4和 24 h细胞凋亡的改变。结果:在术后第24小时,去骨瓣减压组皮质区凋亡细胞比例与对照组相比显著减少(P<0.05和P<0.01),而两组纹状体区凋亡细胞比例在各时间点均无显著性差异(均P>0.05)。结论:皮质区细胞凋亡的减少可能是去骨瓣减压术对局灶性脑缺血保护作用的重要机制之一。

Aim:To investigate the effect of decompressive craniectomy on the changes of apoptosis after focal cerebral ischemia.Methods: Focal cerebral ischemia was induced by using an endovascular occlusion technique. Decompressive craniectomy was performed at insult side 6 h after ischemia. The proportion of apoptotic cells in TUNEL staining in both cortex and striatum was calculated at 4,8,16,24 h after decompression. And the percentage of apoptotic cells was directly detected with flow cytometry at 4 and 24 h after the decompressive procedure.Results:Comparing with non-decompressive group, the percentage of apoptotic cells in the cortex of the decompressive group was found to be decreased significandy at 24 h (P < 0.05 and P < 0.01). No significant difference was found in the striatum with the both methods ( P > 0.05). Conclusion: Decompressive craniectomy could prevent apoptosis in the ischemic cortex 24 h after operation, which was responsible for the fact that decompressive craniectomy could alleviated ischemic damage.