侧柏叶水煎液对糖尿病模型小鼠的治疗作用及作用机制探讨

The therapeutic action of water decoction of platycladus orientalis on diabetes in mice and its related mechanism

目的探讨侧柏叶水煎液对糖尿病模型小鼠的治疗作用及其机制。方法健康成年昆明种小鼠随机分为正常对照组、糖尿病模型组和侧柏叶组。分别记录体重、饮水量、摄食量和粪尿量,血糖仪测定空腹血糖、ELISA法测定血胰岛素水平、羟胺法和TBA法分别测定血清超氧化物歧化酶(SOD)和丙二醛(MDA)的含量,并作肝脏病理形态学观察。结果与正常对照组相比,糖尿病模型组灌胃2周生理盐水后体重明显减轻,饮水量、摄食量和粪尿量明显增加(P<0.05)。与糖尿病模型对照组相比,小鼠灌胃2周侧柏叶水煎液后侧柏叶组体重明显升高,饮水量、摄食量和粪尿量明显降低(P<0.05)。与正常对照组相比,糖尿病模型对照组灌胃2周生理盐水后空腹血糖、血胰岛素水平、胰岛素抵抗系数、血清MDA水平明显升高,血清SOD含量明显降低(P<0.05),肝脏脂肪变性明显,但与糖尿病模型组相比,侧柏叶组空腹血糖、血胰岛素水平、胰岛素抵抗系数、血清MDA水平明显降低(P<0.05),血清SOD水平明显升高(P<0.05),肝脏脂肪变性程度明显减轻。结论侧柏叶水煎液能明显减轻糖尿病小鼠的"三多一少"的症状,对糖尿病有一定的治疗作用,其机制与其抗氧化、降低胰岛素抵抗有关。

Objective To study the therapeutic action of water decoction of platycladus orientalis on diabetes in mice and its related mechanism. Methods Healthy adult Kunming mice were divided randomly into normal control group,diabetes model control group and platycladus orientalis group. In the mice of each group,the weight,amount of water intake,food intake,and excrement were recorded during study and the shape of hepatocytes was observed by HE staining. Fasting blood glucose( FBG) was measured by glucometer. Fasting blood insulin( FINS) was measured by ELISA method. The hydroxylamine method and TBA method were used respectively to measure the blood content of superoxide dismutase( SOD) and malondialdehyde( MDA),respectively. Results Compared with normal control group,The weight of diabetes control group was lost obviously after gavage of normal saline for 2 weeks( P < 0. 05),and the amount of water intake,food intake and excrement were increased evidently( P < 0. 05). Compared to the diabetes model control group,the weight of platycladus orientalis group was increased obviously( P < 0. 05),and the amount of water intake,food intake and excrement were decreased evidently( P < 0. 05). Compared with the normal control group,FBG,FINS,insulin resistance index and blood MDA content of diabetes model control group were increased evidently( P < 0. 05). The blood content of SOD was decreased obviously( P < 0. 05),and the liver fat degeneration oc-curred obviously. Compared with the diabetes model control group,FBG,FINS,insulin resistance index and blood MDA content of platycladus orientalis group were decreased evidently( P < 0. 05),and the blood content of SOD was increased apparently( P < 0. 05). The degree of liver fat degeneration was remitted evidently. Conclusion The water decoction of platycladus orientalis remitted the symptoms of'three polys and one little'obviously caused by diabetes. So it has therapeutic action to diabetes in mice and its mechanism may be related to its anti-oxidative stress and reducing the insulin resistance.