牛血清白蛋白温敏凝胶给药系统的制备及释放度考察

Study on the preparation and release properties of bovine serum albumin loaded temperature-sensitive gel

目的制备牛血清白蛋白(BSA)温敏凝胶给药系统,并考察给药系统的释放情况。方法以温敏凝胶材料普朗尼克F127为载体,以相变温度为指标,通过倒置试管法筛选普朗尼克F127的浓度。通过BCA蛋白定量试剂盒法测定BSA的含量。以PBS缓冲液为释放介质,考察凝胶给药系统中BSA的释放。结果浓度为20%(w/v)的普朗尼克F127凝胶的相变温度为23℃,载入BSA后相变温度不变。BSA在普朗尼克F127凝胶系统中的释放12h可释放80%以上,具有缓释效果,且释放接近零级动力学过程。结论 BSA-普朗尼克F127温敏凝胶系统制备方法简单,具有明显的缓释效果。

Objective To study on the preparation of bovine serum albumin( BSA) loaded temperature-sensitive gel and the release property of BSA. Methods Pluoronic F127 was selected as carriers. The concentration of pluoronic F127 was optimized through the inverted test tube method using the phase-transition temperature as the index. The concentration of BSA was determined using BCA kits. The release property of BSA was investigated using PBS as the dissolution media. Results The phase-transition temperature of pluoronic F127 with the concentration of 20%( w/v) was23℃ and retained identically when BSA was loaded. The results of release showed that after 12 h,more than 80% of BSA was released and the release property was nearly zero-order kinetics. Conclusion The preparation technique of BSA loaded pluoronic F127 temperature-sensitive gel is feasible. The release of BSA could be well controlled.