PLK1和DNAPKcs在弥漫大B细胞淋巴瘤中的表达及临床意义

The expression and clinical significance of PLK1 and DNAPKcs in diffuse large B-cell lymphoma

目的探讨PLK1和DNAPKcs在弥漫大B细胞淋巴瘤(DLBCL)中的临床意义及对DLBCL细胞增殖的影响。方法收集50例DLBCL患者的临床资料,并通过对其病理组织标本进行免疫组化检测PLK1和DNAPKcs的表达水平,分析它们的表达相关性及与DLBCL临床病理特征之间的关系。通过siRNA干扰PLK1和DNAPKcs,采用EdU实验观察DLBCL细胞增殖能力的变化。敲减PLK1的表达,Western blot实验检测DLBCL细胞DNAPKcs的表达变化。结果 PLK1和DNAPKcs表达水平在DLBCL病人和两种细胞系FARAGE和OCI-Ly3中显著高于正常人外周血单核细胞(P<0.05),促进DLBCL肿瘤细胞的增殖,与Ann Arbor分期、患者是否出现B症状、IPI评分正相关(P<0.05),与DLBCL患者年龄、性别无相关性;敲减PLK1后DNAPKcs的表达水平显著降低。结论 PLK1和DNAPKcs可作为DLBCL诊断和预后判断的重要指标。

Objective To study the expression and clinical significance of PLK1 and DNAPKcs in diffuse large B cell lymphoma(DLBCL).Methods The expression of PLK1 and DNAPKcs was detected by immunohistochemistry in 50 DLBCL patients The correlation of the expression to the clinicopathological parametors of DLBCL was analyzed.The effects of PLK1 and DNAPKcs on the DLBCL cell proliferation were observed by EdU staining.The expression of DNAPKcs was determined by Western blot Results The expression levels of PLK1 and DNAPKcs in DLBCL patients and two cell lines of FARAGE and OCI-Ly3 were significantly higher than that in normal human peripheral blood mononuclear cells,positively related to Ann Arbor staging,B symptoms and IPI scores,not to age and gender of DLBCL patients(P>0.05).PLK1 and DNAPKcs promoted the proliferation of OCI-Ly3 cells,the expression level of DNAPKcs was significantly decreased after PLK1 knockdown(P<0.05).Conclusion PLK1 and DNAPKcs would be an important indicator for the diagnosis and prognosis of DLBCL.