钛银合金及其表面纳米管化的生物相容性

Biocompatibility of titanium silver alloy and its surface nanotubes

目的分析纳米钛银合金纳米管复合物的生物相容性。方法首先采用熔融法制备钛银合金(3%的银含量),然后通过阳极氧化技术在钛银合金表面制备纳米管。采用扫描电镜实验方法观察复合物的表面形貌。采用钛银合金及钛银合金纳米管和商业钛金属分别与成骨细胞共同培养方法,扫描电镜下观察细胞在支架上的黏附、增殖及凋亡。采用细胞毒性实验(MTT)实验方法检测金属的相容性。结果通过改变阳极氧化条件能制备不同表面形貌的钛银合金纳米管,将钛银合金及钛银合金纳米管和商业钛金属分别与成骨细胞共同培养3天后,倒置显微镜观察金属材料周围的成骨细胞生长情况,发现钛银合金纳米管及钛银合金周围细胞增殖与商业钛金属无明显差异。同时通过扫面电镜观察细胞在金属材料上的生长增殖情况,细胞在钛银合金纳米管上生长良好,可见大量细胞伪足附着于其上。同时通过细胞毒性实验(MTT),发现钛银合金纳米管及钛银合金与商业钛金属之间无明显差异。结论钛银合金纳米管具有良好的生物相容性。

Objective To analyze the biocompatibility of nano-titanium-silver alloy nanotube composites.Methods Titanium silver alloy(3%silver content)was prepared by melt method.Then,nanotubes were prepared on the surface of titanium-silver alloy by anodizing technique.The surface morphology of the composite was observed under scanning electron microscopy.Titanium silver alloy and titanium silver alloy nanotubes and commercial titanium metal were co-cultured with osteoblasts,respectively,and the adhesion,proliferation and apoptosis of the cells on the scaffold were observed.The compatibility of metals was examined using a cytotoxicity test(MTT)experimental method.Results Titanium-silver alloy nanotubes with different surface topography were prepared successfully by changing the anodizing conditions.After titanium-silver alloy and titanium-silver alloy nanotubes and commercial titanium metal were co-cultured with osteoblasts for 3 days respectively,the growth of osteoblasts around the metal material was observed by an inverted microscope,and the cell growth around the titanium-silver alloy nanotubes and the titanium-silver alloy was found,with no significant difference from commercial titanium.The cells grew well on the titanium-silver alloy nanotubes,with a large number of cellular pseudopods attached to them.MTT showed that there was no significant difference between titaniumsilver alloy nanotubes and titanium-silver alloys and commercial titanium.Conclusion Titanium-silver alloy nanotubes have good biocompatibility.