miR-379对胶质瘤U87MG细胞增殖、迁移和侵袭能力的影响

The effects of miR-379 on the proliferation, migration and invasion ability of glioma U87MG cells

目的研究miR-379对胶质瘤U87MG细胞增殖、迁移和侵袭能力的影响。方法 Real-time PCR方法检测miR-379在胶质瘤U87MG细胞和正常星型胶质细胞中的表达水平。在胶质瘤U87MG细胞中瞬时转染miR-379 agomir并用real-time PCR方法验证其转染效率。CCK-8方法检测miR-379对胶质瘤U87MG细胞增殖能力的影响。划痕实验检测miR-379对胶质瘤U87MG细胞迁移能力的影响。Transwell侵袭实验检测miR-379对胶质瘤U87MG细胞侵袭能力的影响。结果 miR-379在胶质瘤U87MG细胞中的表达水平显著低于在正常星型胶质细胞中的表达。miR-379 agomir抑制胶质瘤U87MG细胞的增殖、迁移和侵袭能力。结论 miR-379抑制胶质瘤U87MG细胞的增殖、迁移和侵袭能力。

Objective To study the effects of miR-379 on the proliferation,migration and invasion ability of glioma U87 MG cell.Methods The expression level of miR-379 in glioma U87 MG cells and normal astrocytes was detected by real time PCR.Instantaneous transfection of miR-379 agomir in glioma U87 MG cells was verified by real-time PCR.The effects of miR-379 on proliferation ability of glioma U87 MG cells were detected by CCK-8 assay.The effects of miR-379 on the migration ability of glioma U87 MG cells were detected by would-healing assay.The effects of miR-379 on the invision ability of glioma U87 MG cells were detected by the Transwell invision assay.Results The expression level of miR-379 in glioma U87 MG cells was significantly lower than that in normal astrocytes.miR-379 inhibits the proliferation,migration and invasion ability of glioma U87 MG cells.Conclusion miR-379 inhibits the proliferation,migration and invasion ability of glioma U87 MG cells.