培养条件对养殖真鲷弧菌病病原菌毒素产生的影响

EFFECTS OF CULTURAL CONDITIONS ON THE PRODUCTION OF TOXIN BY VIBRIO MIMICUS

研究了温度、酸碱度、气体环境、营养成分及培养时间等培养条件对真鲷病原菌最小弧菌Vm Pm毒素产生的影响。结果表明 ,温度对Vm Pm毒素的产生有显著影响 ,在 30℃培养时产量最高 ;当pH在弱碱性环境( 7.2左右 )时 ,Vm Pm毒素产量最高 ;振荡通气能极大地促进Vm Pm毒素的产生 ,显著提高其产量 ;Vm Pm毒素的产量随着培养时间的延长而逐步增加 ,到 36h即可达到最高水平 ,并维持至 72h ;改良海水营养培养基与普通海水营养培养基相比 ,更有利于毒素的产生 ,毒素产量可提高 1倍。

In this article, effect of cultural conditions such as temperature, pH, air conditions, culture medium and incubation time on the yield of toxin Vm-Pm by Vibrio mimicus were studied. Temperature had significant influence on the toxin Vm-Pm production, the optimal temperature for toxin Vm-Pm production was 30℃, and the optimal initial pH for the toxin Vm-Pm production was pH 6—8. The yield of toxin Vm-Pm was significantly enhanced when growing the strain with shaking. The yield of toxin Vm-Pm was the maximal during the mid- and late-logarithmic growth phases (when the strain was incubated for 36 hours), and was stable during the stationary growth phase(incubated for 72 hours). The yield of toxin in the improved medium containing 0.1% Fe 2+ was twice more than that in the ordinary marine water agar, the result demonstrated that the availability of free iron in the medium had a significant influence on the production of toxin Vm-Pm,and also the pathogenesis of Vibrio mimicus.