牙鲆淋巴细胞转化和细胞免疫

THE LYMPHOCYTES TRANSPLANT TEST AND CELL-MEDIATED IMMUNE OF PARALICHTHYS OLIVACEUS

利用淋巴转化实验测试了抗原和有丝分裂原对牙鲆T淋巴细胞的影响 ,结果显示在适宜的生长温度范围内 ,低温延缓或阻止牙鲆细胞免疫应答的发生 ,导致淋转水平下降。外周血和脾淋巴细胞非特异性淋转水平较前肾高 ,外周血和脾淋巴细胞的淋转水平没有显著差异。这可能和外周血、脾和前肾中T细胞占淋巴细胞的比例有关。外周血较脾和前肾特异性淋转水平高 ,而前肾的淋巴细胞转化水平较脾高。注射引起牙鲆应激反应导致淋转水平升高。

To determine the effect of foreign antigen and mitogen on immune function of tlymphocytes of Paralichthys olivaceus, lymphocytes proliferation induced by haemoglutinin(PHA) and IgG at 16℃,18℃,20℃,22℃ and 24℃ were detected.The proliferation rate of lymphocytes from different flounder lymphoid were analyzed.Lymphocytes obtained from peripheral blood,spleen and head kidney were collected for PHA-stimulated and IgG-stimulated proliferation assay and their proliferation was determined with MTT.Morphology of P.olivaceus lymphocytes after treated with PHA were observe by microscope,we found lymphocytes,lmyphoblasts and mitogenesis in the culture system.The results showed that the proliferation of lymphocytes were decreased sharply at 24℃,within the suitable cultural temperature,from 16℃ to 22℃ in our experiment,at the comparatable low temperature the proliferation of cultured cells were decreased.As compared with those of spleen and head kidney,the proliferation rate of peripheral blood lymphocytes is very high.The rates show no difference between spleen and head kidney.Both PHA and IgG can induce the proliferation of lymphocytes.The nonspecific lymphocyts proliferation(Induced by PHA) of peripheral blood and spleen was significantly higher than that of the lymphocyte from head kidney(p<0.05),there is no diference between those of blood and spleen (p>0.05).The specific lymphocytes (Induced by IgG)proliferation of peripheral blood were higher than those of head kidey and spleen(p< 0.05),and the head kidney lymphocyte proliferation was higher than spleen's(p<0.05).Injection increased the proliferation of lymphocytes of P.olivaceus.