大鼠三叉神经脊束间质核接受内脏和躯体伤害性信息的calbindin D-28k神经元向臂旁核的投射

Calbindin D-28k-containing neurons receiving visceral and somatic nociceptive information in interstitial nucleus of the spinal trigeminal tract project to the parabrachial nuclei in the rat

应用荧光金 (FG)逆行束路追踪结合Fos和calbindinD 2 8k(CB)免疫荧光组织化学三重标记法 ,观察了大鼠三叉神经脊束间质核 (INV)接受口面部皮肤和上消化道伤害性信息的CB神经元向臂旁核 (PB)的投射。结果显示 ,口周刺激组FG逆标细胞和Fos免疫反应阳性细胞主要分布于注射和刺激同侧INV的背侧边缘旁核 (PaMd)和三叉旁核 (PaV) ;大量的CB免疫阳性细胞分布于双侧INV。同侧INV内FG逆标细胞中有 77 3 %呈CB免疫反应阳性 ,40 7%呈Fos免疫反应阳性。在FG和CB双标记的神经元中 ,又有一部分 (约 3 8 5 % )为FG/CB/Fos三标细胞。上消化道刺激组的FG逆标细胞、CB免疫阳性细胞和FG/CB双标细胞的数量和分布与口周刺激组相似 ,但Fos免疫阳性细胞分布于双侧的INV。在同侧INV ,FG/Fos双标细胞占FG逆标细胞总数的 41 9% ,FG/CB/Fos三标细胞占FG/CB双标细胞的 5 2 0 %。以上结果提示 ,INV直接投射到PB的CB神经元接受口面部皮肤和上消化道的伤害性信息 。

The calbindin D 28k (CB) containing neurons in the interstitial nucleus of the spinal trigeminal tract (INV) that receive visceral and orofacial somatic nociceptive information and emanate projections to the parabrachial nuclei (PB) were investigated by the triple labeled methods of fluorogold (FG) retrograde tracing combined with Fos and CB proteins immunofluorescence histochemistry in the rat. The results showed (1) in the perioral stimulation group, a large number of FG retrograde labeled and Fos immunoreactive neurons were found in the paratrigeminal nucleus (PaV) and the dorsal paramarginal nucleus (PaMd) of the INV ipsilateral to FG and formalin injection made to the PB and lips, respectively, while a lot of CB immunoreactive neurons were distributed in the INV bilaterally; (2) a majority of the FG retrograde labeled neurons (77 3%) were double labeled with CB, and 40 7% of them were double labeled with Fos; about 38 5% of FG/CB double labeled neurons were FG/CB/Fos triple labeled in the INV; and (3) in the upper alimentary tract stimulation group, the distribution and the numbers of FG retrograde labeled, CB immunoreactive neurons and FG/CB double labeled neurons in the INV were similar to those of the perioral stimulation group as described above, except that the Fos immunoreactive neurons were distributed in the INV bilaterally, approximately 41 9% of the FG retrograde labeled neurons were FG/Fos double labeled, and over half (52 0%) of those double labeled neurons were FG/CB/Fos triple labeled. The results indicate that a part of CB containing neurons in the INV receive orofacial somatic and visceral nociceptive information and that these neurons sent projections directly to the PB. The CB containing neurons might play an important role in the transmission of the peripheral nociceptive information from INV to PB.