青霉素处理分离蓝藻细胞液泡

ISOLATION OF CELL VACUOLES IN CYANOBACTERIATREATED BY PENICILLIN

分别用 8 2 0 0u/mL ,15 30 0u/mL ,1880 0u/mL和 30 80 0u/mL的青霉素处理培养 4d的 4种蓝藻 :鱼腥藻sp .5 95 (Anabaenasp .5 95 )、念珠藻sp .96 (Nostocsp.96 )、织线藻 2 46 (Plectonemaboryanum 2 46 )和赖氏鞘丝藻〔LyngbyalargerheimiiMab (Gom)〕 ,处理时间为 2 4h ,各藻有不同程度的细胞破裂并且释放液泡 ,液泡释放率分别为 1.80 % ,2 .18% ,0 .48%和 0 .2 8% .图 4表 1参

Four species of Cyanophyta, Anabaena sp.595, Nostoc sp.96, Plectonema boryanum 246 and Lyngbya largerheimii Mab(Gom), were cultured for 4 d and treated with penicillin of 8 200 u/mL,15 300 u/mL,18 800 u/mL and 30 800 u/mL for 24 h, respectively. It was discovered that cells of the tested Cyanobacteria broke in different degrees and vacuoles were released, and the release rates were 1.80%, 2.18%, 0.48% and 0.28%, respectively. Fig 4, Tab 1, Ref 16