几种测定灰树花多糖中蛋白质含量方法的比较研究

Comparative Study on Determination Methods of Residual Protained in Grifolan

用双缩脲法、紫外吸收法、考马斯亮蓝G -2 5 0染色法三种方法对灰树花多糖中蛋白质含量进行测定 ,并同微量凯氏定氮法进行对比。结果表明 :在蛋白质量浓度 0 1～ 1 0mg/mL范围内 ,其测量误差分别为双缩脲法大于 5 0 % ,紫外吸收法大于 30 % ,考马斯亮蓝G -2 5 0染色法最低可小于 3%。考马斯亮蓝G - 2 5 0染色法简单 ,迅速 ,且相对较为准确 ,是测定灰树花多糖中蛋白质含量的有效方法。

The Determination methods of residual proteins contained in grifolan such as Biuret Reaction,UV absorption and the dying method with Coomassie Brilliant Fluka G-250 were studied and compared with the Kjeldahl's minor nitrogendetermining method.The results indicated that the determination deviations are more than 50% for Biuret Reaction,more than 30% for UV absorption method,and less than 3% for the dying method with Coomassie Brilliant Fluka G-250 at least while the concentration of proteins ranges from 0 1 to 1 0 mg/ml.The dying method with Coomassie Brilliant Fluka G-250 possesses the properties of simplicity,rapidness and exactness,so it's an effective method of determining the content of proteins contained in grifolan.