摘要
长裙竹荪 (Dictyophoraindusiata)子实体经 ρ(Na2 CO3 ) =2 %溶液提取 ,用蛋白酶法和Sevag法相结合除去蛋白 ,乙醇分级沉淀 ,级分 2经DEAE -SephadexA -2 5柱层析纯化得到长裙竹荪多糖Di-S2P .经测定该多糖为均一组分 ,分子量约为 8 7× 10 5,红外光谱呈现出典型的多糖吸收峰 ,紫外扫描无核酸和蛋白质的特征吸收峰 .纸层析和气相层析分析得知Di -S2P含有D -葡萄糖、D-半乳糖、D -甘露糖和D -木糖 ,其摩尔比为 1 6 2∶1 87∶1 0 0∶0 93。
A polysaccharide Di-S2P was extracted from the dried fruiting bodies of Dictyophora indusiata by 2% Na 2CO 3 solution.THe extract was deproteinized by proteinase treatment and Sevag method,then precipitated by fractional precipitation with ethanol.Di-S2P was further purified by DEAE-Sephadex A-25 chromatography column.Di-S2P was proved to be homogeneous by gel filtration and polyacrylamide gel electrophoresis.The molecular wight of Di-S2P was estimated to be 870000.No characteristic absorption of nucleic acid and protein was shown in Di-S2P UV spectrum,but typical absorption of polysaccharide was shown in its IR spectrum.The components of Di-S2P were identified by paper and gas chromatographies,which was composed of D-glucose,D-galactose D-mannose,and D-xylose with the molar ratio of 1 62∶1 00∶1 87∶0 93
出处
《中国食用菌》
北大核心
2003年第2期40-42,共3页
Edible Fungi of China
基金
福建省教委资助项目 (JB2 0 0 2 9)
