吲哚丁酸对桉树插条多酚氧化酶的影响及其与生根的关系

Study on the relationship between the changes of activities and isoenzymes of polyphenol oxidase and the rooting of Eucalyptus cuttings after treatment with indolebutyric acid

尾叶桉MLA无性系(简称MLA)为难生根植物,尾叶桉U6无性系(简称U6)和刚果12号桉W5无性系(简称W5)为易生根植物。MLA插条内的PPO活性比U6、W5的低。用吲哚丁酸(IBA)处理桉树的插条后,在扦插生根的不同阶段,插条内的PPO活性呈现规律性的变化。蛋白质含量呈上升趋势。PPO同工酶谱带也随生根的进程出现增多现象。讨论了多酚氧化酶与桉树插条生根的关系。

Eucalyptus urophylla S. T. Blade MLA clone(MLA)was one of the plants which rootings were difficult. E.urophylla S.T.Blade U6 clone(U6)and E.ABL 12 W5 clone(W5)were relatively easytoroot plants. The activity of polyphenol oxidase (PPO) in MLA was lower than that of in U6 and W5. After the cuttings of Eucalyptus were treated with indolebutyric acid (IBA),the activities of PPO and the content of protein increased regularly in different stages of rooting. The isoenzymes of PPO increased after treatment with IBA. The relationship between PPO and rooting of Eucalyptus cuttings was discussed.