摘要
利用携带质粒pBI130 1(含有GUS和NPTII)的根癌农杆菌转化葡萄胚性愈伤组织 ,对比 5种酚类物质对遗传转化效率的影响。结果表明 :阿魏酸与乙酰丁香酮相比 ,GUS点数无显著差异 ,最佳浓度为 15 0mg/L。
In order to find out more efficient and cheaper phenolic compounds insteading of 3,5 dimetho xy 4 hydro xyaceto phenone(AS) to improve efficiency of Agrobacterium mediated transformation into grape,this research utilized Agrobacterium strain LBA4404 carrying pBI1301 with GUS and NPT II to transfer embryogenic callus of grape.The results showed that,compared with AS,trans ferulic acid was better than AS,with the best concentration of 150mg/L; 4 Hydroxy 3, 5 dimethoxy benzoesaure and erucic acid also had a significant efficiency of genetic transformation than control II(phenolic compound free).
出处
《园艺学报》
CAS
CSCD
北大核心
2003年第1期77-78,共2页
Acta Horticulturae Sinica
基金
国家教育部资助优秀年轻教师项目
