西洋梨叶片直接再生体细胞胚

Somatic Embryo Genesis from in Vitro Leaves of Pear

以西洋梨‘红安久’和‘绿安久’两个品种的无菌苗幼叶为试材 ,研究了不同培养基对叶片分化不定梢及体细胞胚的影响。结果表明 :基本培养基的组成是影响叶片分化为不定梢还是体细胞胚的关键因素 ,植物生长物质的种类和水平是影响体细胞胚再生率高低的重要因素。诱导培养基NN6 9附加TDZ 2mg/L及NAA 0 .1mg/L ,体细胞胚再生率最高 ,绿安久达 5 5 .5 % ,红安久为 4 7.3%。NN6 9附加BA5mg/L及NAA 0 .1mg/L ,不定梢再生率最高 ,绿安久达 10 0 % ,红安久为 85 .7%。

Young leaves in vitro of pear ( Pyrus communis L.) cultivars Red D'Anjou and D'Anjou were selected as materials. Effect of media on shoot and somatic embryo regeneration from leaves was studied. Results showed that the composition of basic media was the main factor affecting shoot regeneration or somatic embryo regeneration from leaves; the kind and concentration of exogenous hormone supplemented were important factor to higher frequency regeneration of somatic embryo. In this study, somatic embryo regeneration rate was the highest in the treatment with medium NN69 supplemented with 2 mg/L TDZ and 0.1 mg/L NAA, D'Anjou was 55.5%, and Red D'Anjou was 47.3%. Shoot regeneration rate was the highest on NN69 supplemented with 5 mg/L BA and 0.1 mg/L NAA, D'Anjou was 100%, Red D'Anjou was 85.7%.