不同锑剂对早幼粒细胞白血病凋亡诱导作用比较

Apoptosis of acute promyelocytic leukemia cell NB4 induced by different kinds of antimonials

目的 :通过不同锑剂对早幼粒白血病细胞株NB4细胞的凋亡诱导作用的比较 ,力求找到有效治疗早幼粒细胞白血病锑剂药物 .方法 :采用细胞生长曲线、形态学及NBT(硝基四氮唑蓝 )还原试验判定NB4细胞的生长、分化及功能 ;细胞周期分析和DNA电泳研究细胞凋亡 .结果 :三价锑剂能够诱导早幼粒白血病细胞凋亡 ,且具有时间剂量依赖性 ,而五价锑剂对早幼粒白血病细胞没有凋亡诱导作用 .结论 :三价锑剂能够有效地诱导早幼粒白血病细胞凋亡 ,提示酒石酸锑钾作为临床治疗寄生虫病有效药物 ,可以老药新用 ,有望用于临床治疗早幼粒细胞白血病 .

AIM: To study the effects of different kinds of antimonials on proliferation, differentiation and apoptosis in acute promyelocytic leukemia cell line NB4 cells and to find effective antimonials as an anti APL drug in clinical therapy. METHODS: Proliferation curve, morphology and NBT test were used to determine the proliferation and differentiation of NB4 cells. The apoptosis of NB4 cell was analyzed by DNA fragments and FACS. RESULTS: Trivalent antimonials could induce acute promyelocytic leukemia cell NB4 apoptosis in a dose and time dependent manner, but the pentovalent antimonials had no effect on NB4 cell. CONCLUSION: Trivalent antimonials can effectively induce apoptosis of acute promyelocytic leukemia cell NB4, which suggests that trivalent antimonials including potassium antimonyltartrate, an old anti parasite drug,may be used as a new anti APL drug.