不同来源桑白皮东莨菪内酯含量测定

Determination of scopoletin in Cortex Mori from different sources

目的 研究不同来源桑白皮东莨菪内酯含量和测定方法。方法 采用 HPL C法 ,以 Me OH- H2 O- HAc( 40∶ 6 0∶ 0 .2 5 )为流动相 ,345 nm为检测波长。结果 东莨菪内酯平均回收率为 98.88%,RSD为 1.91%,线性范围为 0 .0 2 5 6～ 0 .12 8μg。不同来源最高含量为 0 .0 173%,最低含量为 0 .0 0 2 %。结论 HPL C测定法简便 ,稳定可靠 ,重复性好 ,灵敏度高。

Object To study the contents and the determinating method of scopoletin in Cortex Mori from different sources.Methods Scopoletin was determined by HPLC.The mobile phase was MeOH-H 2O-HAc (40∶60∶0.25).Detection wavelength was at 354 nm.Results The average recovery was 98.88% with RSD=1.91% and the linear range was 0.025 6—0.128 μg.The highest content of scopoletin was 0.017 3% and the lowest content was 0.002% from different producing areas.Conclusion The method is easy, accurate, reliable with good reproducibility and high sensitivity.