新型抗菌涂层Ag2O-TiO2-NTS在治疗人工关节置换术后假体周围感染在兔的体内外研究

Titanium oxide nanotubes embedded with silver dioxide nanoparticles for treatment of prosthetic joints infections in vivo and in vitro in rabbit

探讨Ag2O-TiO2-NTS在治疗人工关节置换术后假体周围感染的抑菌作用。体外试验:不同浓度的涂层溶液与共培养,并计算菌落数及抗菌率。体内试验:动物随机分组,分别使用Ag2O-TiO2-NTS复合涂层的螺钉及没有涂层的普通螺钉。拆线后分别注入表皮葡萄球菌悬液获金黄色葡萄球菌悬液。各时间点记录肛温、CRP、ESR及PCT。结果表明,各组菌落数及抗菌率两两比较,差异有统计学意义。组内各时间点肛温、CRP、ESR、PCT差异有统计学意义。组间各组指标差异有统计学意义,Ag2O-TiO2-NTS对表面葡萄球菌及金黄色葡萄球菌具有良好的抗菌作用。

The aim of this study was to determine the effects of Ag2O-TiO2-NTs on epidermidis staphylococcus and staphylococcus aureus infections after artifcial joint replacement in vivo and in vitro.In vitro different concentrations of Ag2O-TiO2-NTs solution were co-cultured with epidermidis staphylococcus and staphylococcus aureus,and the colony number and antibacterial rate were calculated.New Zealand rabbits were selected to construct animal models of prosthetic infections.Animals in the experimental group were implanted with nails with Ag2O-TiO2-NTs composite coatings,whereas those in the control group were implanted with common nails.After taking out stitches,epidermidis staphylococcus and staphylococcus aureus supernatant were injected into the affected articular cavity of the knee joint.Anti-infection effects of Ag2O-TiO2-NTs composite coatings were evaluated,including body temperature,CRP,ESR and PCT.Infection rates of prostheses of animals in the experimental group were also lower than the control group(P<0.05).Measured values of body temperature,CRP,ESR,and PCT of animals in the experimental groups were remarkably lower than in the control groups,at different time points after bacterial inoculation(all P<0.05).Artifcial joint prostheses with novel antibacterial coatings,made from Ag2 O-TiO2-NTs composites have better performance regarding anti-epidermidis staphylococcus and staphylococcus aureus infections.