菊科植物青蒿细胞色素P450基因家族分析

Bioinformatics analysis of cytochrome P450 monooxygenases gene family from Artemisia annua

从NCBI中下载了青蒿P450全部全长序列,经过一致性鉴定,共获得41个P450单加氧酶基因。根据P450标准命名规则,这41个基因分布在3个基因簇的11个家族中。与水稻、拟南芥P450氨基酸序列比对发现,拟南芥中所特有的CYP82、CYP83和CYP716 3个家族在青蒿中出现,而水稻特有的CYP92家族在双子叶植物青蒿中也存在。对青蒿P450蛋白质的理化性质和结构特性进行了分析,二级结构分析结果表明,青蒿P450蛋白质的二级结构以α-螺旋为主。

In the study, a total of 41 genes encoding cytochrome P450 monooxygenases were identified based on the seguence downloaded from NCBI. These genes were classified into 3 clans and 11 families by sequence similarity. One of the first interesting features of the Arabidopsis species?specific families CYP82, CYP83 and CYP716, and family CYP92 specif?ically in rice are present in Artemisia annua.The secondary structure of putative protein P450 is characterized by α?helix.