摘要
为了探究蛇床子素对稻瘟病菌致病力的影响,采用生长速率和致病力测定的方法对其进行研究。室内生长速率测定结果表明,蛇床子素对稻瘟病菌的生长有抑制作用,32μg/ml浓度下,菌丝生长开始受到抑制,160μg/ml浓度下,抑制率达到65%左右;且液体培养状态下,抑制作用更加明显。扫描电镜观察发现,60μg/ml蛇床子素处理条件下,菌丝呈现畸形,菌丝表面出现干瘪及皱缩,部分菌丝出现断裂等现象。致病性试验结果表明,20μg/ml蛇床子素可降低稻瘟病菌对水稻的致病力,40μg/ml蛇床子素可完全抑制稻瘟病菌的侵染;同时附着胞形成试验发现,20μg/ml蛇床子素能够部分抑制附着胞的形成,40μg/ml蛇床子素则能完全抑制孢子的萌发。
The influence of osthol on the pathogenicity of Magnaporthe oryza was studied by detecting the growth rate and morphology of mycelia. The colony growth was inhibited when treated with 32μg/ml osthol and the inhibition rate was about 65% when treated with 160 μg/ml osthol. The scanning electron microscope revealed clear morphological alteration ( abnormality,wizening and fracture) of mycelia of M.oryzae with 60μg/ml osthol treatment.The pathogenicity was reduced by the application of 20μg/ml osthol,and was totally lost with 40μg/ml osthol.Appressorium formation was partially inhibi?ted with 20 μg/ml osthol and the conidia could not germinate at all with 40 μg/ml osthol.
出处
《江苏农业学报》
CSCD
北大核心
2015年第6期1265-1269,共5页
Jiangsu Journal of Agricultural Sciences
基金
江苏省青年基金项目(BK20140749)
