鹅输卵管TLR家族基因差异表达的研究

Differential gene expressions of Toll-like receptors in goose oviduct

本试验旨在研究已公开的10种鸟类Toll样受体(TLRs)家族基因在鹅输卵管不同功能部位的差异表达。分别采用RT-PCR和q RT-PCR技术检测输卵管基因表达谱和表达水平。结果显示除TLR1-1和TLR5分别在膨大部和子宫部组织无表达外,其他TLR家族基因在输卵管不同部位均表达,其中4个基因在阴道部高表达,2个基因分别在伞部和峡部高表达,1个基因分别在膨大部和子宫部高表达;TLR2-2和TLR21在输卵管不同功能部位间表达差异不显著(P>0.05);阴道部TLR1-1表达显著高于伞部(P<0.05),TLR3表达显著高于伞部、峡部和膨大部(P<0.05),TLR5表达显著高于伞部和膨大部(P<0.05),TLR15表达显著高于子宫部(P<0.05);伞部TLR1-2和TLR2-1表达显著高于峡部和阴道部(P<0.05);峡部TLR4表达显著高于子宫部和阴道部(P<0.05),TLR5显著高于伞部和膨大部(P<0.05);膨大部TLR2-1表达显著高于峡部和阴道部(P<0.05);子宫部TLR7表达显著高于伞部、峡部、膨大部和阴道部(P<0.05)。表明鹅输卵管不同功能部位差异化表达TLR,存在抵御病原微生物侵染的非特异性免疫反应机制。

This study aims to investigate the gene expression of Toll?like receptors ( TLRs) in different segments of goose oviduct. The profiles and levels of TLRs gene expressions in all segments of goose oviduct were examined by RT?PCR and qRT?PCR, respectively. Except for non?detectable TLR1?1 and TLR5 expressions in magnum and uterus, respectively, the others were all expressed in oviduct. A?mong 10 TLRs genes, 4 were highly expressed in vagina tissue, 2 in infundibulum and isthmus tissues, respectively, and 1 in magnum and uterus tissues, respec?tively. Gene expressions of TLR2?2 and TLR21 differed in?significantly in various segments of oviduct ( P>0?05 ) .&nbsp;Compared with gene expression in vagina, TLR1?1 mRNA expression in infundibulum, TLR3 mRNA expressions in infun?dibulum, isthmus and magnum, TLR5 mRNA expressions in infundibulum and magnum, and TLR15 mRNA expression in uterus were significantly decreased ( P<0?05 ) . Compared with gene expression in infundibulum, TLR1?2 and TLR2?1 mRNA expressions in isthmus and vagina were significantly decreased (P<0?05). TLR4 and TLR5 mRNA expressions in isthmus were higher than those in uterus and vagina and those in infundibulum and magnum, respectively. TLR2?1 mRNA expression in magnum was higher than those in isthmus and vagina, and TLR7 mRNA expression in uterus was higher than those in the other tissues. In conclusion, differential expression of Toll?like receptors genes may play essential roles in ovi?ductal innate immunity for host defense.