摘要
为了在原核细胞中表达金黄色葡萄球菌弹性纤维结合蛋白(EBPS),制备多克隆抗体,将构建的p ET30a-n EBPS重组质粒转化大肠杆菌BL21(DE3),经IPTG诱导表达后,亲和层析法纯化重组蛋白,以纯化的重组蛋白为抗原免疫新西兰大耳白兔制备多克隆抗体,ELISA方法检侧抗体滴度,亲和层析法纯化兔抗n EBPS多克隆抗体。结果显示,利用p ET30a-n EBPS重组质粒,经原核表达和亲和层析获得了n EBPS蛋白;利用n EBPS蛋白质制备多克隆抗体,间接ELISA检测的纯化抗体效价可达0.686(OD450值),P/N值达6.7。
To prepare polyclonal antibody of Staphylococcus aureus surface protein nEBPS, the plasmid pET30a?nEBPS was transformed into Escherichia coli BL21 (DE3). IPTG induction were performed and recombinant protein was purified by nickel affinity chromatography. The purified nEBPS protein was used to raise rabbit nEBPS protein antibody. The enzyme?linked immunosorbent assay ( ELISA ) showed that the antibody titers against nEBPS was up to 0?686 ( OD450 ) , with P/N value of 6?7.
出处
《江苏农业学报》
CSCD
北大核心
2015年第6期1430-1434,共5页
Jiangsu Journal of Agricultural Sciences
基金
国家自然科学基金项目(31260600)
内蒙古自治区科技厅科技创新引导计划项目[20111802(2012年
2013年)]
内蒙古自治区自然科学基金项目(2012MS0415)
