鸡FSHβ、ESRα基因多态性及其合并基因型与产蛋性能的关联性分析

Effects of single and combined genotypes of FSHβ and ESRα genes on laying performance in chicken

使用SSCP技术检测FSHβ、ESRα的SNPs位点,分析鸡FSHβ、ESRα基因多态性及其合并基因型与产蛋性能的相关性,为寻找玫瑰冠鸡产蛋性能相关分子标记提供依据。结果表明:在玫瑰冠鸡和海兰褐蛋鸡群体中分别检测到FSHβ(A-173G)和ESRα(G-78416T、A-119854T、C-158689G)4个多态位点,其中A-173G位点、A-119854T位点、C-158689G位点AB型对玫瑰冠鸡300日龄产蛋数有显著影响(P<0.05);ESRα基因单倍型GTC个体的开产体质量和300日龄产蛋数高于单倍型GAG,是2种鸡的优势基因型;FSHβ、ESRα合并基因型中玫瑰冠鸡H6型的初生质量显著高于H1基因型(P<0.05),海兰褐蛋鸡H1型的开产蛋质量显著高于H9、H10及H12合并基因型(P<0.05),开产日龄显著早于H11基因型(P<0.05),300日龄产蛋数显著高于其他合并基因型(P<0.05)。因此FSHβ(A-173G)、ESRα(A-119854T)、ESRα(C-158689G)AB型与FSHβ、ESRα合并基因H6型有望作为与玫瑰冠鸡产蛋性能相关的分子标记。

To analyze the association of FSHβ and ESRα genes' polymorphisms and their combined genotypes with chicken laying performance,so as to provide the basis for searching molecular markers related to laying performance,the SSCP technique was used to detect the SNPs of FSHβ and ESRα.Four polymorphic sites of FSHβ (A-173G) and ESRα (G-78416 T,A-119854 T,C-158689G) were detected in rose cockscomb chicken and hy-line brown hens,in which the AB genotype of loci A-173 G,C-158689 G and C-158689 G showed significant effects on laying eggs in 300-day-old rose cockscomb chicken (P < 0.05).ESRα 's haplotype GTC,which is the dominant genotype of two kinds of chicken,were significantly higher than haplotype GAG in puberty body weight and egg number at 300-day-old.The combined genotype H6 of rose cockscomb chicken was significantly higher than genotype H1 in birth weight.The combined genotype H1 of hy-line brown hens was significantly higher than H9,H10 and H12 (P < 0.05) in primary egg weight,earlier in first egg than H11,and more in egg number than other combined genotypes at 300-day-old (P < 0.05).Therefore,the AB genotype of ESRα,FSHβ (A-173G) and ESRα (A-119854T) and the combined genotype H6 of FSHβ-ESRα are expected to be molecular markers related to laying performance in rose cockscomb chicken.