睡莲品种墨宝自交结实率低的细胞学机理

Cellular mechanisms of low self-fertility of water lily variety Almost Black

睡莲品种墨宝(Nymphaea Almost Black)的自交结实率低,为了找出其原因,本研究以墨宝为材料,进行细胞学研究。研究结果表明,墨宝的花粉活力为4.2%,授粉后12 h,平均每个柱头上萌发的花粉量只有27.2粒。此外,授粉后5 d,27.6%的子房内形成了球形胚,授粉后15 d,只在14.7%的子房内观察到胚胎,授粉后20 d,在6.4%的子房内观察到正常胚胎。以上结果显示,受精前的低花粉活力和柱头上花粉萌发异常以及受精后的胚胎败育是导致墨宝自交结实率低的主要原因。

To find out the reason of low self-fertility of water lily variety Almost Black,cytological study was conducted.The results showed that the pollen viability of Almost Black was only 4.2%.Stigmatic pollen germination was 27.2grains per stigma at 12 h after pollination.The rate of normal embryos was 27.6% at five days after pollination,14.7% at15 days after pollination,and 6.4% at 20 days after pollination.These results suggested that low pollen viability,low stigmatic pollen germination and post-fertilization embryos abortion may be the main factors responsible for low self-fertility of Almost Black.