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青海芥菜型油菜多室基因Bjln2的初步定位 被引量：1

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摘要 多室油菜具有多室多粒的特征,是提高油菜产量的一个有效途径。利用青海芥菜型多室油菜的BC4分离群体对油菜多室基因Bjln2进行初步定位,对分离群体的性状分离比进行卡方检测,检测结果符合1∶1,表明多室性状遗传稳定。利用扩增片段长度多态性(amplified fragment length polymorphism,AFLP)与分群分析法(bulk segregate analysis,BSA)相结合的方法共筛选获得3个与目标基因紧密连锁的标记。连锁遗传分析的结果表明,3个AFLP标记均位于基因Bjln2的同侧,其中SA03MC16距Bjln2基因最近,遗传距离约为13.1 c M;SA14MC13距Bjln2基因最远,遗传距离约为23.5 c M;图谱总长23.5 c M,标记间平均图距为7.8 c M。研究结果为多室基因的精细定位及图位克隆奠定了基础。
作者 陈翠萍 肖麓 赵志刚 杜德志
机构地区 青海大学农林科学院春油菜研究所/青海省春油菜遗传改良重点实验室/青海省高原作物种质资源创新与利用重点实验室
出处 《江苏农业科学》 北大核心 2015年第9期32-35,共4页 Jiangsu Agricultural Sciences
基金 国家"863"计划(编号:2011AA10A104) 青海省重点实验室发展专项(编号:2014-Z-Y11)
关键词 青海省 芥菜型油菜 多室 基因定位 扩增片段长度多态性 分群分析法
分类号 S565.4 [农业科学—作物学]
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参考文献9

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二级参考文献10

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江苏农业科学
2015年 第9期

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