淀粉酶活性常量与微量测定方法比较

Comparison between the Methods for Constant Measurement and Micro Determination of Amylase Activity

以实验室保存的淀粉酶产生菌枯草芽孢杆菌为出发菌株,经过平板分离、点种、碘熏蒸,筛选出产淀粉酶活力较高的菌株。本实验重点采用常量测定法和微量测定法对酶活性进行测定,并对所得数据进行比较分析,结果显示,两种方法测定的酶活性差异不明显,说明微量测定法是可以代替常量测定法的。

The bacterium bacillus subtilis which was produced from amylase and kept in the laboratory was treated as the starting strain. The strain with higher production of amylase was screened through the flat panel separation,dibbling and iodide fumigation. This experiment used the constants determination and the micro determination to determine the amylase activity and the data was analyzed by comparative analysis. The result showed that the difference of two methods for the determination of enzyme activity was not apparent,which proved that micro determination method could replaced the constant determination method.