摘要
以水产品中的硝基呋喃类代谢物残留为研究对象,建立超高效液相色谱-串联质谱(UPLC-MS/MS)测定水产品中硝基呋喃类代谢物残留分析方法。结果显示:硝基呋喃类代谢物在1. 0～100. 0 ng/mL的范围内线性关系良好,相对回收率为86. 2%～108%,相对标准偏差为3. 4%～9. 6%,检出限为0. 25μg/kg,定量限为0. 5μg/kg。与以往采用的超高效液相色谱-串联质谱方法相比,新建方法操作简单、快速、准确,是定量检测水产品中硝基呋喃类代谢物残留的有效技术手段。
Based on the residue of nitrofuran metabolites in aquatic products,a super high performance liquid chromatography-tandem mass spectrometry(uplc-ms/MS)was established to determine the residue of nitrofurans metabolites in aquatic products.The results were good in the range of 1~100.0 ng/mL,and the relative recovery rate was 86.2~108%,the relative standard deviation was 3.4~9.6%,and the detection limit was 0.25μg/kg,and the quantitative limit was 0.5μg/kg.Compared with the ultra high performance liquid chromatography-tandem mass spectrometry method,the new method is simple,rapid and accurate,which provides effective and practical technical means for quantitative detection of nitrofuran metabolites in aquatic products.
作者
李剑
陈泽暖
蔡楠
LI Jian;CHEN Ze-nuan;CAI Nan(Guangdong Food and Drug Vocational College,Guangzhou 510520,Guangdong,China;South China Sea Fisheries Research Institute,Guangzhou 510897,Guangdong,China)
出处
《江苏调味副食品》
2019年第1期34-37,44,共5页
Jiangsu Condiment and Subsidiary Food
基金
基于计算流体力学的复杂食品管网系统中生物被膜的形成与调控研究基金项目"超高效液相色谱-串联质谱法快速检测水产品中硝基呋喃类代谢物残留"(31401591)
关键词
硝基呋喃类代谢物
残留分析
高效液相色谱-串联质谱
优化
nitrofuran metabolites
residual analysis
high performance liquid chromatography-tandem mass spectrometry
optimization
