摘要
牛皮肤成纤维细胞经血清饥饿或预激活处理后获得核胞体 ,并注入去核卵母细胞内构建重组胚。检查重组胚 2 4h和 36h卵裂率以及 8d囊胚率 ,以评估供体细胞及其处理方法对体细胞核移植效果的影响。实验结果表明 :来自 3个年龄 (6、 18和 36月龄 )、 2个品系 (红安格斯肉牛和荷斯坦奶牛 )的 4头供体牛皮肤细胞重组胚的卵裂率和囊胚率均无差异。生长到完全汇合的 36月龄荷斯坦牛供体细胞血清饥饿 10～ 13d组重组胚的 36h卵裂率显著低于 0d (对照 )、 3～ 5d和 6～ 9d组 ,囊胚率显著低于 3～ 5d组 ;经 5 μmol/L离子霉素或7%乙醇预激活 5min重组胚的卵裂率和囊胚率均与对照组无差异。
This study was designed to assess the effects of the sources of the donor cells and the treatments to the cells on the development of nuclear transferred embryos in vitro .Fibroblast cells of bovines were or not treated with serum starvation or preactivation,and then nuclei were gained.Reconstructed embryos (RE) were produced by microinjecting the nuclei into the cytoplasm of enucleated oocytes,of which the cleavage rate (CR) was checked at 24 and 36 h,and the blastocyst rate (BR) on D8.The experiment resulted in:①There was no difference in CR and BR of RE among three aged (6,18 and 36 months) animals and two breeds (Angus and Holstein) for four individuals in total.②CR of RE at 36 h,which produced with full confluent fibroblast cells of 36 month Holstein experienced for 10-13 d serum starvation duration,was lower than those for 0 d (control),3-5 d and 6-9 d;BR of RE was only lower than that for 3-5 d.③CR and BR of RE,which produced from cells of 36 month Holstein,had no difference between the control and the preactivation by 5 μmol/L ionomycin or 7% ethanol.
基金
国家高技术研究发展计划资助项目 (A2 0 0 1AA2 13 0 81)
